All Episodes

Displaying episodes 31 - 60 of 98 in total

5 Controls for Immunofluorescence: A Beginner’s Guide

#68 — Controls are fundamental to getting meaningful data. Especially in long, drawn-out experiments like immunofluorescence imaging. In this episode, we explain 5 typ...

What is Water of Crystallization? Everything You Need to Know

#67 — What are those mysterious extra water molecules at the end of some chemical names? Do they matter to your experiments? And what should you do when they aren't wa...

Better Plasmid Purification: 11 Reasons Your Plasmid Yield is Low

#66 — Are your plasmid yields low? Not sure what is considered a good yield? Need help boosting the amount of plasmid you get from your preps? In this episode, we disc...

The Importance of Delegation for Scientists

#65 — Delegation is not just for managers! No matter where you are in your research career, there is an opportunity to delegate. In this episode, we discuss how you ca...

How To Make ECL Reagent: 4 Ingredients, Better Blots

#64 — Like many experiments, doing a Western blot typically means comparing half a dozen online protocols, failing completely, triple-checking the recipes of your more...

3 Hot Tips to Optimize Your Western Blot Transfers

#63 — Are you fed up with Western blots that look like your pen leaked in your pocket? We all are. That's why we've put together our three favorite tips for better Wes...

Top 5 of the Most Commonly Used Cell Lines

#62 — Do you want to use a cell line but are unsure where to start? Or perhaps you’re just curious about the most commonly used cell lines. Listen to our top 5 most co...

Get a Grip: Dealing with Sweaty Glove Hands

#61 — Fed up with fighting to put on another pair of gloves with sticky hands? Faced the embarrassment of giving a sweaty handshake? You need to listen to our top tips...

What Ponceau S Staining Can Reveal About Your Western Blot

#60 — There's nothing quite as frustrating as imaging a western blot only to find your bands are a wonky mess, your blot is covered in bubbles, or your bands are missi...

Plasmid Incompatibility: Should You Care?

#59 — Have you heard of plasmid incompatibility? Should you be worried about it? Listen to this episode of Mentors At Your Benchside to discover exactly what plasmid i...

Protein Colocalization: 2 Essential Methods to Prove Protein Overlap

#58 — One of the most common goals in biology is to prove that two proteins colocalize. Usually, colocalization happens as part of a crucial disease pathway or poorly ...

Why Do Enzymes Have Optimum Temperatures?

#57 — Some microbes survive at 37°C, and some survive at 90°C. So why do their respective enzymes have optimum temperatures? Listen to this episode and learn about the...

The How and Why of Removing Serum From Your Media

#56 — Do you add serum, such as fetal bovine serum, to your cell culture medium? This ingredient may be critical for cell growth and proliferation, but it could negati...

Cell Passage Number Explained

#55 — Do you know how to keep track of cell passages using passage numbers? Is there a maximum passage number you should grow cells? And is there a better way to keep ...

10 Common PPE Sins

#54 — Do some people's terrible PPE habits bug you? Are you guilty of not wearing your PPE in the lab?Listen to our top 10 PPE sins. Sins that we've all definitely com...

PPE for Eye and Face Protection—Better Safe than Sorry!

#53 — Anywhere but the face! On the list of places you wouldn't want to spill a chemical, I bet your face ranks pretty high.Wise scientists know their face protection....

How to Handle Strong Acids in the Lab

#52 — Acids are ubiquitous in the lab. We use them for many tasks, such as pH-ing solutions, cleaning glassware, calibrating instruments, and dissolving analytes.But t...

How to Use a pH Meter Correctly in 4 Simple Steps

#51 — A neglected pH meter means less reliable experiments, poor reproducibility, and time wasted. Yet, despite being critical to all our experiments, they are frequen...

The Dangers of UV Light in the Lab: Keep You and Your Experiments Safe

#50 — While we all know the dangers of too much UV light from the sun and the need to wear sunscreen, how much thought do we give to the dangers of UV light in the lab...

Problems Amplifying GC-rich regions? 5 Easy Solutions

#49 — It's not easy being rich. If your DNA is GC-rich and you're struggling to amplify it, you aren't alone. Listen to this Mentors At Your Benchside episode to disco...

8 Steps to More Successful Experiments

#48 — One of the critical skills required for any scientist is the ability to consistently design and carry out successful experiments. It’s also vital that any experi...

How Buffers Work—A Guide For Biologists

#47 — Buffers are everywhere in a lab. But do you know why we use them? Listen to this episode of Mentors At Your Benchside as we explain what buffers are, how they wo...

What Is a Ct Value?

#46 — If you are performing quantitative PCR (qPCR), you must understand what a Ct value is. In this episode of Mentors at Your Benchside, we'll take you through its c...

Different DNA Polymerases Explained

#45 — Taq polymerase is a fantastic all-rounder for standard PCRs, but what do you do if you need something a bit more specialist? In this episode of Mentors At Your B...

10 Good and Evil Ways to Use Your Dr Title

#44 — So you might be wondering why you bothered with all those years of failed experiments, committees, overbearing supervisors, thesis writing, and thesis defending....

How DNA Extraction Kits Work in the Lab

#43 — DNA extraction kits. A magic black (or red or blue) box that helps you isolate DNA without you even thinking. That's great—until things go wrong. Then you have t...

Funny Fruit Fly Gene Names

#42 — There are a lot of humorless scientists out there. But thankfully, some do their bit to make science a bit more fun by adding a splash of imagination to the name...

How to clean and calibrate your lab balance

#41 — Keeping your lab balance clean and calibrated will make your bench work a lot easier. In this episode we give you useful tips to maintain your balance, literally...

How to Write a Thoughtful Discussion for Your Scientific Paper

#40 — Are you struggling to write the discussion section of your paper? It can be daunting to put your results in context and to thoughtfully consider limitations and ...

How Using Oil Immersion Microscopy Can Increase Your Resolution

#39 — Oil immersion microscopy can help increase your resolution in light microscopy. Which, in turn, can lead to better images; and who doesn’t want nicer microscopy ...