Better Plasmid Purification: 11 Reasons Your Plasmid Yield is Low
#66 — Are your plasmid yields low? Not sure what is considered a good yield? Need help boosting the amount of plasmid you get from your preps?
In this episode, we discuss what is a good and bad yield, why your plasmid preps might be sub-optimal, and how you get better yields.
Read the original article for an easy protocol for growing unsaturated cultures. [1] Discover the differences between DNA precipitation and ethanol vs. isopropanol in our article [2] and watch Eppendorf's webinar on centrifugation basics. [3]
Resources:
1. Better Plasmid Purification: 11 Reasons Your Plasmid Yield is Low. Available at: https://bitesizebio.com/13514/boost-plasmid-yield/
2. DNA Precipitation: Ethanol vs. Isopropanol. Available at: https://bitesizebio.com/2839/dna-precipitation-ethanol-vs-isopropanol/
3. Essentials in Centrifugation - Better Safe than Sorry! Available at: https://events.bitesizebio.com/essentials-in-centrifugation-1/
In this episode, we discuss what is a good and bad yield, why your plasmid preps might be sub-optimal, and how you get better yields.
Read the original article for an easy protocol for growing unsaturated cultures. [1] Discover the differences between DNA precipitation and ethanol vs. isopropanol in our article [2] and watch Eppendorf's webinar on centrifugation basics. [3]
Resources:
1. Better Plasmid Purification: 11 Reasons Your Plasmid Yield is Low. Available at: https://bitesizebio.com/13514/boost-plasmid-yield/
2. DNA Precipitation: Ethanol vs. Isopropanol. Available at: https://bitesizebio.com/2839/dna-precipitation-ethanol-vs-isopropanol/
3. Essentials in Centrifugation - Better Safe than Sorry! Available at: https://events.bitesizebio.com/essentials-in-centrifugation-1/
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