#64 — Like many experiments, doing a Western blot typically means comparing half a dozen online protocols, failing completely, triple-checking the recipes of your more suspicious buffers, then remaking them and starting again anyway.

To ease the pain, this episode gives you a reliable ECL reagent recipe so you can prepare your own fresh (and private) stash anytime you need. We also explain what all the components do.

Check out the corresponding article to see a mechanism for the oxidation of luminol and links to papers discussing alternative enhancers. [1] If you want better Western blot transfers, read our top three tips. [2] And better yet, we've put this ECL reagent recipe, an adaptable Western blot protocol complete with buffer recipes, and loads of troubleshooting tips together in a cheat sheet to stick in your lab book. Download it for free here. [3]

Resources:
1. How To Make ECL Reagent: 4 Ingredients, Better Blots. Available at: https://bitesizebio.com/8970/how-to-make-your-own-ecl/
2. 3 Hot Tips to Optimize Your Western Blot Transfers. Available at: https://bitesizebio.com/7356/optimize-your-western-blot/
3. Bitesize Bio's Western Blot Protocol Cheat Sheet. Available at: https://bitesizebio.com/western-blot-protocol/