All Episodes
Displaying episodes 1 - 30 of 110 in total
Let’s Talk About Stats: Methods for Comparing Two Sets of Data
#110 — Comparing two sets of data is a fundamental process in statistical analysis, crucial for drawing meaningful conclusions across various fields. Whether it's for ...
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Personal Advice on Building Your Professional Network. It Takes a Village
#109 — How do you build a scientific network that gives you the best chance of getting your research funded? How can you identify who to include in your network, and h...
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4 Fixatives for Histology and Cytometry: Perfect Your Preservation
#108 — What should you use to fix your cells? Alcohols or aldehydes? Gluteraldehyde or formaldehyde? And how long will your cells stay fixed?This episode explains the ...
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Microscope Disinfection: A Quick Guide
#107 — Need to give your microscope a quick clean to get rid of some grime but unsure what cleaning agent to use? Have you had a nasty sample on there recently and nee...
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Tissue Processing For Histology: What Exactly Happens?
#106 — Transforming a tissue sample into a slide ready for microscopic exploration involves a series of critical steps. Among these, tissue processing is a fundamental...
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Multiple Fragment Ligation: The Why and How
#105 — You may be familiar with standard single fragment ligations: insert, vector, ligase—done! But what if you have a complex cloning project with a massive region o...
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Funding Opportunities and the Flow of Money in Science
#104 — What funding stream is right for you? Industry or government? Non-profits or crowdfunding? It depends on what you're researching, but also where you want to tak...
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Maxam–Gilbert Sequencing: What It Is and 3 Modern Applications
#103 — DNA sequencing is a fundamental technique in modern molecular biology that has revolutionized the study of genes.In the old days, Maxam–Gilbert sequencing was t...
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Confocal Laser Scanning Microscopy Explained In 3 Easy Steps
#102 — Fluorescence microscopy images not only look great but also allow us to get a better understanding of cells, structures, and tissues. And confocal laser scannin...
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How to Become an Expert at Getting Funded
#101 — Discover what it takes to become an expert at getting funded, from simple habits such as summarizing what you read in the literature, to big steps such as orga...
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5 Types of Difficult Lab Supervisor and How to Handle Them
#100 — Science attracts so many different and quirky personalities that you are bound to have some people you just don’t get along with. Conflicts happen, and there ar...
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7 Top Tips to Make the Most of Your Flow Cytometry Training
#99 — So you’ve got your flow cytometry training booked and are one step closer to that precious data.But if you want to hit the ground running and get some useful dat...
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What Reagents Can You Use Past Their Chemical Expiry Date?
#98 — Our labs can contain thousands of chemicals, many of which will be past their given expiry date and many of which are expensive to buy and replace. Replacing the...
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How to Write an Effective Research Interest Statement
#97 — A research interest statement is essential to successfully apply for an academic job. In this episode, we delve into how to craft an outstanding one. [1]We cover...
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How to Preserve Microorganisms: Store Your Cells Better
#96 — An appropriate microorganism preservation method can make all the difference in maintaining the viability of your microbial strains because it plays a crucial ro...
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Overhang PCR: Add Missing DNA Sequences Using Primers
#95 — Have you ever accidentally forgotten to add the Kozak consensus sequence to the start of a coding gene? Or forgotten to include the stop codon? Did you clone som...
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Practical Applications and Considerations of Phenol-Chloroform Extraction
#94 — While there are lots of methods to choose from for cleaning up your RNA or DNA samples, for many researchers, phenol-chloroform is the go-to technique. In this e...
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How to Become a Bioinformatician
#93 — Bioinformatics is an interdisciplinary field that combines mathematics, computer science, physics, and biology to help answer key questions in modern biological ...
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8 Cell Lysis Methods to Break Cell Walls
#92 — We all need to lyse cells to extract the goodness—our samples—from them.However, there are many cell lysis methods. Some are harsh, while some are gentle. Some a...
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Genetic Variants Explained
#91 — Genomes are complex and encode a vast quantity of information. One of their key features is genetic variants—aberrations in the genetic sequence, usually in the ...
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How to Identify Supercoils, Nicks and Circles in DNA Plasmid Preps
#90 — Are you confused about the banding pattern of DNA on agarose gels? DNA can take many structural forms depending on its source and how you have isolated and purif...
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Simplicity in Science: How to Increase your Research Effectiveness by Doing Less
#89 — "Achieve more by doing less" sounds like a piece of cheap advice, but there is a lot of wisdom in it. Research is complicated. You must choose the best questions...
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How to Totally Nail Your First in situ Hybridization
#88 — Getting the best out of your in situ hybridizations requires choosing the correct protocol, deciding if sections or whole mount is better, using the right equipm...
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Choosing The Right Blood Collection Tubes
#87 — Selecting the right blood collection tubes for your experiment is crucial. But do you know what tubes to use for which type of blood sample?In this episode, we c...
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A Step-by-Step Guide to Designing qPCR Primers
#86 — qPCR primer design is a bit of science, a bit of magic, and a little bit of luck. In this episode, we cover the science of qPCR primer design, a cornerstone in c...
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How to Passage Cells in Culture
#85 — Working with living cells is a tricky business, and tiny fluctuations in environmental conditions can affect their physiology and impact your experiments. Or wor...
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3 Easy Tips for Avoiding Measurement Drift in Analytical Balances
#84 — Every experiment starts by preparing some buffer solutions. And every buffer solution starts with weighing out some compounds on an analytical balance. But these...
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How to Make Your Own Chemically Competent Cells
#83 — Chemically competent cells are a key resource in molecular biology labs. But do you really understand what is meant by chemically competent?In this episode of Me...
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5 Easy Tips for Keeping Your Centrifuge Alive
#82 — Did you know that most centrifuge accidents result from user error and improper centrifuge care? While proper balancing of samples is important, it is not the on...
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How Blunt-End Cloning Works
#81 — You’re probably aware of the two main types of restriction cloning (sticky-end and blunt-end cloning), but do you know the difference? And do you know how to do ...
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