How Blunt-End Cloning Works

#81 — You’re probably aware of the two main types of restriction cloning (sticky-end and blunt-end cloning), but do you know the difference? And do you know how to do both?

In this episode, we share what blunt-end cloning is, how it works, and give you tips and tricks for performing blunt-end cloning.

Visit the original article for detailed diagrams and helpful tables, [1] brush up on how DNA ligase works, [2] and reacquaint yourself with the different DNA polymerases. [3] You can also get help to improve your blunt end ligations, [4] and discover the magic of TA cloning. [5]

1. A Beginner’s Guide to How Blunt-End Cloning Works. Available at:
2. DNA Ligation: How it Works & 6 Top Tips.  Available at:
3. Get To Know Your DNA Polymerases. Available at:
4. 10 ways to improve blunt-end ligations. Available at:
5. Ta-Da! The Magic of Taq and TA Cloning. Available at:

Creators and Guests

Dr. Laura Grassie
Dr. Laura Grassie
Content Creation Manager, Bitesize Bio
How Blunt-End Cloning Works