How to Passage Cells in Culture

#85 — Working with living cells is a tricky business, and tiny fluctuations in environmental conditions can affect their physiology and impact your experiments. Or worse, it can lead to their death. Game over!

Passaging your cells involves removing them from their growth medium to transfer them to fresh vessels with fresh media.

In this episode, learn how to passage adherent and suspension cells while minimizing the impact on them. See the different passaging strategies you can adopt and decide which ones are right for you!

Check out the corresponding online article for handy tables and figures. [1] Learn all about cell confluency and why it matters [2] and explore the critical components of cell culture media. [3]

1. How To Passage Cells in Culture. Available at:
2. How Confluent Are Your Cells? A Beginner’s Guide to Measuring Cell Culture Confluency. Available at:
3. What’s in Your Cell Culture Medium? Available at:

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How to Passage Cells in Culture