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Dr. Adam Pawson
Senior Managing Editor, Bitesize Bio
Appears in 18 Episodes
Isoelectric Focusing: A Simple Way to Enhance Your Protein Separation
#112 — Isoelectric focusing (IEF) is a powerful technique distinct from the more familiar SDS-PAGE, [1,2] tailored for separating proteins or peptides based on their i...
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Let’s Talk About Stats: Methods for Comparing Two Sets of Data
#110 — Comparing two sets of data is a fundamental process in statistical analysis, crucial for drawing meaningful conclusions across various fields. Whether it's for ...
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Tissue Processing For Histology: What Exactly Happens?
#106 — Transforming a tissue sample into a slide ready for microscopic exploration involves a series of critical steps. Among these, tissue processing is a fundamental...
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How to Write an Effective Research Interest Statement
#97 — A research interest statement is essential to successfully apply for an academic job. In this episode, we delve into how to craft an outstanding one. [1]We cover...
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Overhang PCR: Add Missing DNA Sequences Using Primers
#95 — Have you ever accidentally forgotten to add the Kozak consensus sequence to the start of a coding gene? Or forgotten to include the stop codon? Did you clone som...
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How to Become a Bioinformatician
#93 — Bioinformatics is an interdisciplinary field that combines mathematics, computer science, physics, and biology to help answer key questions in modern biological ...
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8 Cell Lysis Methods to Break Cell Walls
#92 — We all need to lyse cells to extract the goodness—our samples—from them.However, there are many cell lysis methods. Some are harsh, while some are gentle. Some a...
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Choosing The Right Blood Collection Tubes
#87 — Selecting the right blood collection tubes for your experiment is crucial. But do you know what tubes to use for which type of blood sample?In this episode, we c...
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A Step-by-Step Guide to Designing qPCR Primers
#86 — qPCR primer design is a bit of science, a bit of magic, and a little bit of luck. In this episode, we cover the science of qPCR primer design, a cornerstone in c...
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A Beginner’s Guide to Hematoxylin and Eosin Staining
#80 — Dive into the fascinating world of histology as we explore the basics of Hematoxylin and Eosin (H&E) staining, a cornerstone technique in tissue study. [1]Whethe...
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5 Ingredients for the Perfect Protein Purification Buffer
#79 — Are you struggling to keep your proteins "happy" and active for your experiments? In this episode of Mentors At Your Benchside, we dive into the five critical el...
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8 Steps to More Successful Experiments
#48 — One of the critical skills required for any scientist is the ability to consistently design and carry out successful experiments. It’s also vital that any experi...
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Does Your h-index Measure Up?
#37 — How do you measure how good you are as a scientist? How would you compare the impact of two scientists in a field? What if you had to decide which one would get ...
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Keep Calm and Spin On: The Whats and Whys of Centrifugation
#30 — We have all heard about centrifugation, or more specifically, the horror stories associated with centrifugation going wrong.But don't let centrifugation scare yo...
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DNA Ligation: How it Works & 6 Top Tips
#27 — Optimal conditions for DNA ligation reactions are a delicate balance between DNA molecules interacting and the enzymatic ligation reaction.In this episode, you'l...
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DNA Precipitation: Ethanol vs. Isopropanol
#12 — As a follow-up to our episode about ethanol precipitation of DNA and RNA [1], this episode explains the differences between DNA precipitation in ethanol and isop...
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Ethanol Precipitation of DNA and RNA: How it Works
#6 — Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acid preparations in an aqueous solution.In this episode, we'll bring ...
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Is it Worth Doing a PhD After a Master’s?
#1 — Is it worth doing a PhD? This is a question that probably plagues every research student at some point in their career.In this episode, we explore 5 important que...
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