#27 — Optimal conditions for DNA ligation reactions are a delicate balance between DNA molecules interacting and the enzymatic ligation reaction.

In this episode, you'll discover our top 6 DNA ligation tips to improve the efficiency of your ligations and increase your cloning success rate!

Read the full article to get more practical advice for optimizing DNA ligation reactions for cloning. [1] Want more cloning advice? Read about Cloning with Compatible Cohesive Ends, [2] and discover how to Remove Unwanted DNA from Vectors. [3] Also, find out why T4 DNA ligase is such a popular ligase and perhaps the only ligation enzyme you’ll ever need. [4]

Resources:
1. DNA Ligation: How it Works & 6 Top Tips. Available at: https://bitesizebio.com/10279/how-dna-ligation-works/
2. Assembling the Puzzle: Cloning with Compatible Cohesive Ends. Available at: https://bitesizebio.com/21716/assembling-the-puzzle-cloning-with-compatible-cohesive-ends/
3. Tech Clinic #1: Removing Unwanted DNA from Vectors — beat Murphy’s Law. Available at: https://bitesizebio.com/10228/removing-unwanted-dna-from-vectors-how-to-beat-murphys-law/
4. T4 DNA Ligase: The Only Ligase You’ll Ever Need? Available at: https://bitesizebio.com/46482/t4-dna-ligase-the-only-ligase-youll-ever-need/