#34 — Whether you like it or not, you need to know the concentration of your protein samples accurately. Binding constants, enzyme kinetics, and activity assays all need accurately-quantified protein to deliver accurate results.

But there's a head-spinning amount of protein quantification assays out there! The BCA assay, Bradford assay, biuret reaction, the Folin–Lowry assay—but hold on.

Which assay is appropriate for your sample and why? In this episode of Mentors At Your Benchside, we break down our top 5 protein quantification assays, go into their advantages and disadvantages, and suggest what types of proteins they are appropriate for.

Be sure to visit the corresponding article for table breakdown of the assays discussed. [1] You can also follow up this podcast by reading our article on how colorimetric assays work. [2]

Resources:
1. Top 5 Protein Quantification Assays. Available at:
https://bitesizebio.com/23824/top-5-protein-quantification-assays/
2. Ask a Chemist: How Colorimetric Assays Work. Available at:
https://bitesizebio.com/7214/ask-a-chemist-how-colorimetric-assays-work/