#25 — Phenol extraction of DNA is a commonly used method for removing proteins from nucleic acids, for example, to remove proteins from cell lysate during genomic DNA preparation.

It's commonly used, but not well understood.

Listen to this episode to get a quick explanation of how phenol extraction of DNA works.

Visit the original article to see handy diagrams on how phenol extraction works. [1] To get more help and advice on DNA extraction, read our related articles and discover how chloroform can clean up your phenol extractions, [2] learn how ethanol precipitation works, [3] and decide if ethanol or isopropanol is best for precipitating your samples. [4]


Resources:
1. The Basics: How Phenol Extraction of DNA Works. Available at: https://bitesizebio.com/384/the-basics-how-phenol-extraction-works/
2. Practical Application of Phenol-Chloroform Extraction. Available at: https://bitesizebio.com/3651/practical-application-of-phenol-chloroform-extraction/
3. Ethanol Precipitation of DNA and RNA: How it Works. Available at: https://bitesizebio.com/253/the-basics-how-ethanol-precipitation-of-dna-and-rna-works/
4. DNA Precipitation: Ethanol vs. Isopropanol. Available at: https://bitesizebio.com/2839/dna-precipitation-ethanol-vs-isopropanol/